UJI ANTIBAKTERI RIMPANG GANDASULI (HEDYCHIUM CORONARIUM) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI DENGAN PERBANDINGAN METODE EKSTRAKSI

Aris Suhardiman

Abstract


Indonesia has various types of plants those have potential as medicine to overcome health problems in line with the expanding of traditional medicine industry. One example of plants from the Zingiberaceae family is Hedychium coronarium, which is not widely known by the community despite it has efficacy as an antibacterial which is not inferior to other Zingiberaceae family. The research was conducted to determine whether the Hedychium coronarium rhizome shown to have antibacterial activity against the growth of Staphylococcus aureus and Escherichia coli, knowing antibacterial compound that contained in Hedychium coronarium rhizome, also knowing the most effective extraction method to take the antibacterial compound from Hedychium coronarium rhizome. The gandasuli rhizome was extracted by maceration and reflux with 96% ethanol. Antibacterial activity test carried out by the agar diffusion method, and the analysis of antibacterial compounds with bioautography, and the results are monitored with vanillin-sulfuric acid. Maceration and reflux extract at a concentration of 90% has antibacterial activity with inhibition zones, each for 8 and 10 mm against Escherichia coli as well as 9 and 10 mm against Staphylococcus aureus. Bioautography results show a positive effect on the appearance of vanillin-sulfuric acid. Reflux extract has better antibacterial activity, while the antibacterial compound is an essential oil group.

 Keywords: Hedychium coronarium, antibacterial, Staphylococcus aureus, Escherichia coli, bioautography.


Full Text:

PDF

References


Agoes, Goeswin. 2007.Seri Farmasi Industri: Teknologi Bahan Alam. Bandung: Penerbit Institut Teknologi Bandung.

Aziz, M. Abdul., et al. 2009. Antibacterial and Cytotoxic Activities of Hedychium coronarium J. Koenig. Research Journal of Agriculture and Biological Science, 5, 969-972.

Brooks, G. F., Janet, S. B. dan Stephen, A. M. 2007. Jawetz, Melnick and Adelbergs, Mikrobiologi Kedokteran Edisi 23 (Alih bahasa oleh Mudihardi, E. et al.). Jakarta: Penerbit Buku Kedokteran EGC.

Castellani dan Chalmers. 1919. Escherichia coli, Taxonomic Serial No: 285 (Diperbaharui Juni 2012). https://www.itis.gov/servlet/SingleRpt/SingleRpt?search_topic=TSN&search_value=285#null.

Chimnoi, N., Pisutjaroenpong, S., Ngiwsara, L., et al. 2008. Labdane Diterpene From The Rhizomws of Hedychium coronarium. Natural Product Research, Volume 22, Nomor 14, 1255-1262.

Ching Ho, Jiau. 2011. Antimicrobial, Mosquito Larvacidal and Antioxidant Properties of The Leaf and Rhizome of Hedychium coronarium. Journal of The Chinese Chemical Society, 58, 563-567.

Choma, I. 2005. The Use of Thin-Layer Chromatography With Direct Biautography for Antimicrobial Analysis. LCGC Europe.

Farnsworth, Norman R. 1966. Biological and Phytochemical Screening of Plants. Journal of Pharmaceutical Sciences, Volume 55, Number 3.

Harborne, J. B. 1987. Metode Fitokimia. Bandung: Penerbit Institut Teknologi Bandung.

Kunnumakkara, A.B., et al. 2008. Coronarin D, A Labdane Diterpene, Inhibits both Constitutive and Inducible Nuclear Factor-Kappa B Pathway Activation, Leading to Potentiation of Apoptosis, Inhibition of Invasion, and Suppression of Osteoclastogenesis. Mol. Cancer Ther. 7, 3306-3317.

Pelczar, M. J. 2005. Dasar-Dasar Mikrobiologi. Jakarta: Penerbit Universitas Indonesia (UI Press).

Pranitha, K., Shalini, K., Pratibha, M., dan Suneha, S. 2014. Hypoglicemic Effect of Ethanolic Extract of Hedychium coronarium Linn Rhizome in Alloxan Induced Diabetes in Rat Model. International Journal of Research in Pharmacology and Pharmacotherapeutics, Volume 3, Issue 3.

Rocenbach. 1884. Staphylococcus aureus, Taxonomic Serial Number: 369 Diperbaharui Juni 2012). https://www.itis.gov/servlet/SingleRpt/SingleRpt?search_topic=TSN&search_value=369#null.

Rudi, I. 2010. Penuntun Dasar-Dasar Pemisahan Analitik. Kendari: Universitas Haluoleo.

Shekhar, Tailor Chandra dan Anju, Goyal. 2015. A Comprehensive Review On Hedychium coronarium J Koenig (Dolanchampa/ Kapurkachri). International Journal Research in Ayurveda and Pharmacy, 6, 98-100.

Stahl, Egon. 1985. Analisis Obat Secara Kromatografi dan Mikroskopi. Bandung: Penerbit Institut Teknologi Bandung.

van Steenis, G. G. G. J. 1987. Flora (Alih bahasa oleh Mosoe S.). Jakarta: Pradnya Paramita.

Wagman, Gerald H. 1983. Chromatography of Antibiotics.

Wijayakusuma, Hembing. 2005. Atasi Kanker dengan Tanaman Obat. Jakarta: Puspa Swara.




DOI: http://dx.doi.org/10.36465/jop.v1i2.326

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Journal of Pharmacopolium

p-ISSN: 2620-8563; e-ISSN: 2621-1521


Index: